mouse anti gh monoclonal antibody  (Santa Cruz Biotechnology)

Journal: bioRxiv

Article Title: Membrane fusion activity of herpes simplex virus 1 glycoproteins from a hyperfusogenic virus

doi: 10.1101/2023.12.04.569993

Figure Lengend Snippet: HSV-1 ANG, KOS, or rid1 were added to Vero cells at 4°C, 15°C, or 37°C for 2 hr and then after attached virus inactivation, cells were incubated at 37°C for 18-24 hr. Virus titers were measured by plaque assay.

Article Snippet: Anti-HSV-1 gH mouse monoclonal antibody BBH1 was purchased from Abcam, and rabbit polyclonal antibody to gH (R137) was from R. Eisenberg and G. Cohen.

Techniques: Virus, Incubation, Plaque Assay

Journal: bioRxiv

Article Title: Membrane fusion activity of herpes simplex virus 1 glycoproteins from a hyperfusogenic virus

doi: 10.1101/2023.12.04.569993

Figure Lengend Snippet:

Article Snippet: Anti-HSV-1 gH mouse monoclonal antibody BBH1 was purchased from Abcam, and rabbit polyclonal antibody to gH (R137) was from R. Eisenberg and G. Cohen.

Techniques:

Journal: bioRxiv

Article Title: Membrane fusion activity of herpes simplex virus 1 glycoproteins from a hyperfusogenic virus

doi: 10.1101/2023.12.04.569993

Figure Lengend Snippet: (A) CHO-K1 cells were transfected with plasmids encoding HSV-1 ANG glycoproteins gB, gC, gD, or gH and gL. Lysates were resolved by SDS-PAGE. Western blots were probed with anti-gB, gC, gD, or gH antibodies. Molecular weight standards (in kilodaltons) are shown to the right. (B) CHO-K1 cells were transfected with plasmids encoding HSV-1 KOS or ANG glycoproteins for 24 hr. Cells were fixed with paraformaldehyde, and then incubated with antibodies specific for gB (cocktail of H126, H1359, and H1817), gC (cocktail of 1C8, 3G9, H1413, and T96), gD (DL6), or gH (BBH1). HRP-conjugated Protein A was added followed by ABTS substrate. OD values ranged from 0.59 to 1.73 for KOS glycoproteins and 0.17 to 0.26 for empty vector. (C) CHO-K1 effector cells transiently expressing HSV-1 ANG gB, gD, gH, gL and T7 polymerase were co-cultured for 18 hr with CHO-nectin-2 cells transiently transfected with a plasmid coding luciferase under control of the T7 promoter and fusion was quantitated by luciferase-induced luminescence. Results are the mean of three independent experiments. * , P < 0.05; ** , P < 0.01, Student’s t test.

Article Snippet: Anti-HSV-1 gH mouse monoclonal antibody BBH1 was purchased from Abcam, and rabbit polyclonal antibody to gH (R137) was from R. Eisenberg and G. Cohen.

Techniques: Transfection, SDS Page, Western Blot, Molecular Weight, Incubation, Plasmid Preparation, Expressing, Cell Culture, Luciferase

Journal: bioRxiv

Article Title: Membrane fusion activity of herpes simplex virus 1 glycoproteins from a hyperfusogenic virus

doi: 10.1101/2023.12.04.569993

Figure Lengend Snippet: CHO-K1 effector cells expressing either HSV-1 KOS or ANG gB, gD, gH, gL and T7 polymerase were co-cultured for 18 hr with (A) CHO-HVEM cells or (B) CHO-K1 cells expressing nectin-1 transfected with the luciferase plasmid. Fusion was quantitated by luciferase-induced luminescence. Luciferase activity of KOS gD, gB, gH, gL was set to 100%. Results are the means of at least two independent experiments. * , P < 0.05, Student’s t test.

Article Snippet: Anti-HSV-1 gH mouse monoclonal antibody BBH1 was purchased from Abcam, and rabbit polyclonal antibody to gH (R137) was from R. Eisenberg and G. Cohen.

Techniques: Expressing, Cell Culture, Transfection, Luciferase, Plasmid Preparation, Activity Assay

Journal: bioRxiv

Article Title: Membrane fusion activity of herpes simplex virus 1 glycoproteins from a hyperfusogenic virus

doi: 10.1101/2023.12.04.569993

Figure Lengend Snippet: CHO-K1 effector cells expressing either HSV-1 KOS or ANG gB, gD, gH, gL and T7 polymerase were co-cultured for 18 hr with (A, B) CHO-K1 cells expressing nectin-1 or (C) CHO-HVEM cells transfected with the luciferase plasmid. Fusion was quantitated by luciferase-induced luminescence. Luciferase activity of KOS gD, gB, gH, gL was set to 100%. Results are the mean of three independent experiments. ns, not significant (Student’s t test).

Article Snippet: Anti-HSV-1 gH mouse monoclonal antibody BBH1 was purchased from Abcam, and rabbit polyclonal antibody to gH (R137) was from R. Eisenberg and G. Cohen.

Techniques: Expressing, Cell Culture, Transfection, Luciferase, Plasmid Preparation, Activity Assay

Journal: Human Vaccines & Immunotherapeutics

Article Title: Cross-reactive humoral immunity of clade 2 Oka and MAV/06 strain-based varicella vaccines against different clades of varicella–zoster virus

doi: 10.1080/21645515.2023.2210961

Figure Lengend Snippet: Image of FAMA test with anti-VZV glycoprotein monoclonal antibodies using six different VZV strains as FAMA antigens (400× magnification). (a) gH, (b) gB, (c) gI, (d) gE.

Article Snippet: Sera were twofold serially diluted with Dulbecco’s phosphate-buffered saline (DPBS, Lonza, Breda, Netherlands), and mouse monoclonal anti-VZV glycoprotein H (gH), gB, gI, and gE antibodies (Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA) were diluted 1:50 in DPBS.

Techniques: Bioprocessing

Journal: Cell Reports Medicine

Article Title: Single-cell sequencing identifies differentiation-related markers for molecular classification and recurrence prediction of PitNET

doi: 10.1016/j.xcrm.2023.100934

Figure Lengend Snippet:

Article Snippet: Mouse monoclonal anti-GH (clonal E−7) , Santa Cruz Biotechnology , Cat#sc-374266; RRID: AB_10989917.

Techniques: Multiplex Assay, Software

Journal: Scientific reports

Article Title: Ghrelin inhibits autonomic response to gastric distension in rats by acting on vagal pathway.

doi: 10.1038/s41598-020-67053-y

Figure Lengend Snippet: Figure 1. Effect of acylated ghrelin on the variation of blood pressure (BP). (A,B) Variation of BP in response to graded gastric distension (GD) of 20, 40, 60, 80 mmHg. A first set of GD (baseline) was followed by intravenous (iv) injection of vehicle (saline; n = 10, A) or acylated ghrelin at 30 µg/kg (n = 10, B), then a second set of GD was performed 5 minutes after injection. (C) Ten minutes before ghrelin administration the Ghrelin receptor antagonist [D-Lys3]-GHRP-6 was intravenously administered (n = 8). Data are expressed as mean ± SEM. **p < 0.01.

Article Snippet: 10-μm frontal sections were preincubated for 1 h with 0.1 M PBS (pH 7.4) containing 0.3% Triton X-100, and 5% fetal bovine serum (FBS), then incubated overnight at 4 °C with mouse monoclonal anti-Ghrelin receptor antibody (1:200, SC-374515, Santa Cruz Biotechnology, Germany), and guinea pig polyclonal anti-ASIC3 antibody (1:250, AB5927, Millipore, USA) or rabbit polyclonal anti-TRPV1 antibody (1:200, ACC-030, Alomone Labs, Israel) diluted in the same buffer.

Techniques: IV Injection, Saline, Injection

Journal: Scientific reports

Article Title: Ghrelin inhibits autonomic response to gastric distension in rats by acting on vagal pathway.

doi: 10.1038/s41598-020-67053-y

Figure Lengend Snippet: Figure 2. Effect of bilateral vagotomy and splanchnectomy on autonomic response to gastric distension following ghrelin administration. Variation of BP in response to graded gastric distension (GD) of 20, 40, 60, 80 mmHg. A first set of GD (baseline) was followed by intravenous (iv) injection of acylated ghrelin at (30 µg/kg i.v) in vagotomized (n = 10, A) and splanchnectomized (n = B) rats. Data are expressed as mean ± SEM.

Article Snippet: 10-μm frontal sections were preincubated for 1 h with 0.1 M PBS (pH 7.4) containing 0.3% Triton X-100, and 5% fetal bovine serum (FBS), then incubated overnight at 4 °C with mouse monoclonal anti-Ghrelin receptor antibody (1:200, SC-374515, Santa Cruz Biotechnology, Germany), and guinea pig polyclonal anti-ASIC3 antibody (1:250, AB5927, Millipore, USA) or rabbit polyclonal anti-TRPV1 antibody (1:200, ACC-030, Alomone Labs, Israel) diluted in the same buffer.

Techniques: IV Injection

Journal: Scientific reports

Article Title: Ghrelin inhibits autonomic response to gastric distension in rats by acting on vagal pathway.

doi: 10.1038/s41598-020-67053-y

Figure Lengend Snippet: Figure 4. Effect of ghrelin on neuronal activation. Phosho ERK 1/2 on total ERK1/2 ratio was assessed by Western Blot in sham (n = 8) and distended rats previously treated with saline (n = 8) or ghrelin (30 µg/kg i.v., n = 12).

Article Snippet: 10-μm frontal sections were preincubated for 1 h with 0.1 M PBS (pH 7.4) containing 0.3% Triton X-100, and 5% fetal bovine serum (FBS), then incubated overnight at 4 °C with mouse monoclonal anti-Ghrelin receptor antibody (1:200, SC-374515, Santa Cruz Biotechnology, Germany), and guinea pig polyclonal anti-ASIC3 antibody (1:250, AB5927, Millipore, USA) or rabbit polyclonal anti-TRPV1 antibody (1:200, ACC-030, Alomone Labs, Israel) diluted in the same buffer.

Techniques: Activation Assay, Western Blot, Saline

Journal: Scientific reports

Article Title: Ghrelin inhibits autonomic response to gastric distension in rats by acting on vagal pathway.

doi: 10.1038/s41598-020-67053-y

Figure Lengend Snippet: Figure 3. Effect of the different treatments and vagotomy on gastric compliance. (A) Percentage of change in intra-balloon air volume between the 2nd and the 1st distension sets.*p < 0,05 (vs. Saline); #p < 0,05 (vs. Ghrelin). 2-way ANOVA (Time, Treatment) followed by Sidak’s multiple comparison post hoc test. (B) Intra- gastric balloon air volume corresponding to distension pressures in vagotomised and naïve rats.

Article Snippet: 10-μm frontal sections were preincubated for 1 h with 0.1 M PBS (pH 7.4) containing 0.3% Triton X-100, and 5% fetal bovine serum (FBS), then incubated overnight at 4 °C with mouse monoclonal anti-Ghrelin receptor antibody (1:200, SC-374515, Santa Cruz Biotechnology, Germany), and guinea pig polyclonal anti-ASIC3 antibody (1:250, AB5927, Millipore, USA) or rabbit polyclonal anti-TRPV1 antibody (1:200, ACC-030, Alomone Labs, Israel) diluted in the same buffer.

Techniques: Saline, Comparison

Journal: Scientific reports

Article Title: Ghrelin inhibits autonomic response to gastric distension in rats by acting on vagal pathway.

doi: 10.1038/s41598-020-67053-y

Figure Lengend Snippet: Figure 5. Expression and phenotype of gastric Ghrelin receptor neurons in nodose ganglia. (A) Gastric neurons were identified by Fluorogold retrotracer injection and percentage on gastric neurons was assessed in left and right nodose ganglia. (B) In situ hybridization against ghsr1a mRNA was performed in nodose ganglia (left panel). Test of the antisense probe (upper right panel) and sense probe (lower right panel) in the hypothalamus. (C) Retrolabeled gastric neurons were stained for Ghrelin receptor and TRPV1 by immunofluorescence. (D) Retrolabeled gastric neurons were stained for Ghrelin receptor and ASIC3 by immunofluorescence. Scale bar = 200 µm.

Article Snippet: 10-μm frontal sections were preincubated for 1 h with 0.1 M PBS (pH 7.4) containing 0.3% Triton X-100, and 5% fetal bovine serum (FBS), then incubated overnight at 4 °C with mouse monoclonal anti-Ghrelin receptor antibody (1:200, SC-374515, Santa Cruz Biotechnology, Germany), and guinea pig polyclonal anti-ASIC3 antibody (1:250, AB5927, Millipore, USA) or rabbit polyclonal anti-TRPV1 antibody (1:200, ACC-030, Alomone Labs, Israel) diluted in the same buffer.

Techniques: Expressing, Injection, In Situ Hybridization, Staining, Immunofluorescence

Journal: Scientific reports

Article Title: Ghrelin inhibits autonomic response to gastric distension in rats by acting on vagal pathway.

doi: 10.1038/s41598-020-67053-y

Figure Lengend Snippet: Figure 6. Gastric distension and administration protocol. (A) After anesthesia, a carotid pressure transducer and an intragastric balloon were implanted in rat. A first set of gastric distension ranging from 20 to 80 mmHg with 20 mmHg increments and 4 min resting intervals between distensions was performed. Each distension lasted 20 s and was repeated twice. After 15 min recovery atropine (1 mg/kg i.v.) was administered then saline or ghrelin (30 µg/kg i.v.). Five minutes after the injection a second set of gastric distension was performed. In the third experiment the Ghrelin receptor antagonist of [D-Lys3]-GHRP-6 (0.3 µg kg i.v.) was injected 10 minutes before ghrelin administration.

Article Snippet: 10-μm frontal sections were preincubated for 1 h with 0.1 M PBS (pH 7.4) containing 0.3% Triton X-100, and 5% fetal bovine serum (FBS), then incubated overnight at 4 °C with mouse monoclonal anti-Ghrelin receptor antibody (1:200, SC-374515, Santa Cruz Biotechnology, Germany), and guinea pig polyclonal anti-ASIC3 antibody (1:250, AB5927, Millipore, USA) or rabbit polyclonal anti-TRPV1 antibody (1:200, ACC-030, Alomone Labs, Israel) diluted in the same buffer.

Techniques: Saline, Injection